The Transformation Of E. Coli - 885 Words

Jack Dildabanian Ms. Buckley Genetics 11/6/15 The Transformation of E. coli using the plasmid GFP Purpose: The purpose of this lab is to observe bacterial growth under various conditions including the transformation of bacteria; to understand how the process of transformation occurs. Background Information: Transformation is the â€Å"process by which the genetic material carried by an individual cell is altered by the incorporation of foreign (exogenous) DNA into its genome† (MedicineNet.com, â€Å"Definition of Genetic transformation†). Transformation in bacterial cells occurs when the cell incorporates DNA into its genetic material. Bacteria cells that have the ability to take up DNA are called â€Å"competent.† In a lab setting, this is encouraged by placing the mixtures of transformation solution and plasmid DNA on ice, then rapidly transferring them to a hot water bath for about fifty seconds, and then placing them back on ice again. This procedure is called heat shock and increases the permeability of the cell membrane to DNA. The agent which the new genetic material is incorporated into is the bacterial plasmid. A plasmid is a circular deoxyribonucleic acid (DNA) molecule that replicates independently of the bacterial chromosome and o ften permits a bacteria to gain resistance to an antibiotic. Recombinant plasmids are those which have DNA from two or more sources incorporated into a single plasmid. To make recombinant plasmids, two different plasmids are cut with the sameShow MoreRelatedGenetic Transformation Of E. Coli1166 Words   |  5 PagesGENETIC TRANSFORMATION OF E. COLI WITH pGLO AS A VECTOR USING THE HEAT SHOCK METHOD Katelyn Brown, L07 Introduction: Escherichia coli is a small, usually harmless bacterium that commonly resides in human intestines. First discovered by Theodor Escherich in 1885, it has become a widely used organism in the science world. 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With this new knowledge and understanding of biology came about Biotechnology. Biotechnology is the process of harnessing the ability of living systems to quickly and inexpensively produce important biological materials. We can see the results of Biotechnology in all areas of our life including the food we eat and the medicine we take. Today, we are usingRead MoreBacterial Transformation : Escherichia Coli ( E. Coli )960 Words   |  4 PagesBacterial transformation is the insertion of a plasmid containing a gene or genes of importance into bacteria. It can be used to make large quantities of a promising gene. Bacterial Transformation can be artificial or natural. A plasmid is a small, circular, double-stranded DNA molecule that is separate from a cell s chromosome DNA. Plasmids exist in bacterial cells and they also appear in some eukaryotes. The genes carried in plasmids offer bacteria with genetic benefits, like resistance to antibioticsRead MoreThe Transformation Of : Gfp And E. Coli As A Result Of Pglo1117 Words   |  5 PagesTHE TRANSFORMATION OF GFP AND E. COLI AS A RESULT OF pGLO INTRODUCTION One of the most imperative functions in maintaining the development of evolution is the frequency of genetic transformation: the injection of foreign DNA into another organism’s DNA. This term is defined by the actions of a vector, but more specifically by the actions of plasmids and phages. However, in this experiment we are primarily focused on the effect of the pGLO plasmid transformation of GFP on the E. coli bacteriaRead MoreGenetic Transformation Has Occurred Within The E. Coli Culture975 Words   |  4 PagesThe first step of this experiment was to determine if genetic transformation has occurred within the E. coli culture. The plates that were compared in this section can be seen in Figure I. The plates that were compared were labeled LB+ amp (+) and LB + amp (-). The positive control was the untransformed cell in LB+ amp(-). This control was testing: if the antibiotic would kill all of the untransformed cells in the LB+ amp (+). In figure I. the plate labeled LB + amp (-) had no bacteria on it. ThereforeRead MoreGenetic Transformation : Effects Of Introducing The Gfp Gene Into E. Coli Bacteria1255 Words   |  6 PagesLife 102 L 17 2 December 2014 Genetic Transformation: Effects of Introducing the GFP Gene into E. Coli Bacteria Introduction: When a bacterial cell incorporates foreign DNA into its own genome, this is known as bacterial transformation, a form of genetic transformation. There are several ways to transform genetic material; projectile bombardment, electroporation, and heat shock. This experiment uses the heat shock method. Interest in genetic transformation began as early as 1928 when Frederick GriffithRead MoreBacteria Transformation in Biotechnology Essay1673 Words   |  7 PagesAbstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLOâ„ ¢ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media, it could be seen that theRead MoreGene Expression And Its Engineering1634 Words   |  7 PagesIntroduction Transformation is the process of allowing a cell to be able to accept a gene into itself and allow the gene to become part of the cell s whole, thus changing its form and expressing the gene s function. This process was first discovered by the British bacteriologist Frederick Griffith, who was able to transform Streptococcus pneumoniae into a different strain of Strep. in 1928 (1928). This discovery was pivotal in the way we understand gene expression and its engineering. An exampleRead MoreDifferent Methods, Bacteria, And Development Transduction, Transduction And Transformation Transduction934 Words   |  4 Pagestransduction and transformation Transduction is when a bacteriophage inserts it s DNA into the host bacteria cell, while conjugation is when the bacteria shares DNA through direct contact, eg. horizontal gene transfer. The third method, transformation, is the intake of external DNA into the cell (Chen and Dubnau, 2004). In order for bacteria to be able to take in extraneous DNA, they must first be in a state of competent (Hanahan, 1983). There are two types of transformation cells in transformation: natural